PowerTrack SYBR Green Master Mix contains Taq DNA polymerase, which is tightly controlled through an antibody-mediated hot-start mechanism, helping prevent undesirable early activity of the polymerase at low temperatures that can lead to nonspecific amplification. The yellow sample buffer aids in reaction setup, but is not required to obtain superior results with PowerTrack SYBR Green Master Mix.įormulated for maximum specificity and reproducibility The optional use of this yellow sample buffer results in a reaction mixture that changes from blue to green upon addition of the sample, helping one avoid pipetting mistakes. A separate sample buffer is provided that contains an inert yellow dye. PowerTrack SYBR Green Master Mix contains an inert blue dye that does not interfere with real-time PCR but provides increased visibility in the tube or well. Formulated with UNG/dUTP to prevent contamination of carryover PCR products.Specific and tight reproducibility in Ct’s over a broad dynamic range.Broad primer Tm and concentration compatibility allows qPCR reaction setup flexibility with minimal optimization.Built-in visual indicators aid in reaction setup.Coupled with user-supplied primer sets and template, PowerTrack SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.įeatures of PowerTrack SYBR Green Master Mix include: PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.
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